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R1385AP  Porcine IgG F(c) secondary antibody

1 mg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Porcine IgG F(c) -

Product Description

Rabbit anti Porcine IgG F(c) -

Properties

PresentationAP
HostRabbit
Catalog NumberR1385AP
Quantity1 mg
PriceUS$ 375
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1385AP.pdf

Datasheet Extract

Polyclonal Antibody to Swine IgG F(c) -AP-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenSwine IgG F(c) fragment.
Format
State: Liquid (sterile filtered) purified IgG fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:500 - 1:2,500.
Western Blot: 1:200 - 1:2,000.
Immunohistochemistry: 1:200 - 1:1,000.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, Swine IgG F(c) and Swine Serum.
No reaction was observed against Swine IgG F(ab')2.
StorageStore the antibody as an undiluted liquid at 2-8°C before opening.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
References1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.