R1386AP Porcine IgG F(ab')2 secondary antibody
see all 7 Porcine IgG F(ab')2 products
1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Porcine IgG F(ab')2 -
Product Description
Rabbit anti Porcine IgG F(ab')2 -
Properties
| Presentation | AP |
| Host | Rabbit |
| Catalog Number | R1386AP |
| Quantity | 1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1386AP.pdf |
Datasheet Extract
| Polyclonal Antibody to Swine IgG F(ab')2 -AP- | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Swine IgG F(ab')2 fragment. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). Reconstitution: Restore with 0.5 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: ELISA: 1:1,000-1:3,000. Western Blot: 1:500-1:2,000. Immunohistochemistry: 1:200-1:1,000. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, Swine IgG F(ab') 2 and Swine Serum. No reaction was observed against Swine IgG F(c). |
| Storage | Store the antibody (undiluted) at 2-8°C before opening.
DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: One year from despatch. |
| References | 1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
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