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R1387T  Goat IgG (H&L) Fab Fragment secondary antibody

1 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Donkey anti Goat IgG (H&L) Fab Fragment -

Product Description

Donkey anti Goat IgG (H&L) Fab Fragment -, Presentation: TRITC

Properties

PresentationTRITC
HostDonkey
Catalog NumberR1387T
Quantity1 mg
PriceUS$ 305
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1387T.pdf

Datasheet Extract

Fab Fragment of //Donkey anti-Goat IgG (H&L) -TRITC-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenGoat IgG whole molecule.
Format
State: Lyophilized Fab fragments.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Tetramethylrhodamine isothiocyanate (TRITC; Molecular Weight 444 daltons).
Absorptionemission: 550 nm / 570 nm
Molarratio: 1.2 moles TRITC per mole of Donkey IgG Fab.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum.
No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
StorageStore vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
References1. J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.