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R1388F  Human IgG (H&L) Fab Fragment secondary antibody

1 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Human IgG (H&L) Fab Fragment

Product Description

Goat anti Human IgG (H&L) Fab Fragment , Presentation: FITC

Properties

PresentationFITC
HostGoat
Catalog NumberR1388F
Quantity1 mg
PriceUS$ 305
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1388F.pdf

Datasheet Extract

Concentration1.0 mg/ml (UV absorbance at 280 nm)
ImmunogenHuman IgG whole molecule

Format
State: Lyophilized Fab fragment
Purification: Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorptionemission: 495 nm / 528 nm
Molarratio: 1.8 moles FITC per mole of Goat IgG Fab
Reconstitution: Restore with 1.0 ml of deionized water or equivalent.
ApplicationsSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.
No reaction was observed against anti-Papain or anti-Goat IgG F(c).
StorageStore vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
ReferencesThe and Feltkamp, Immunology 18; 865, 1970. (Conjugation)