R1389F Mouse IgG (H&L) Fab Fragment secondary antibody
1 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Mouse IgG (H&L) Fab Fragment
Product Description
Goat anti Mouse IgG (H&L) Fab Fragment , Presentation: FITC
Properties
| Presentation | FITC |
| Host | Goat |
| Catalog Number | R1389F |
| Quantity | 1 mg |
| Price | US$ 305 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1389F.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml ( by UV absorbance at 280 nm) |
| Immunogen | Mouse IgG whole molecule
|
| Format | State: Lyophilized Fab fragment Purification: Immunoaffinity chromatography BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorptionemission: 495 nm / 528 nm Molarratio: 1.6 moles FITC per mole of Goat IgG Fab Reconstitution: Restore with 1.0 ml of deionized water or equivalent. |
| Applications | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions: Flow cytometry 1:2,000 - 1:10,000 Immunofluorescence microscopy 1:500 - 1:2,500. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
| Storage | Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | The and Feltkamp, Immunology 18; 865, 1970. (Conjugation) |
