R1393B Guinea Pig IgG (H&L) F(ab')2 Fragment secondary antibody
1 mg / US$ 345
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Guinea Pig IgG (H&L) F(ab')2 Fragment
Product Description
Goat anti Guinea Pig IgG (H&L) F(ab')2 Fragment , Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Goat |
| Catalog Number | R1393B |
| Quantity | 1 mg |
| Price | US$ 345 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1393B.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Guinea Pig IgG whole molecule. |
| Format | State: Lyophilized F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer. Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
| Storage | Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
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