R1393HRP Guinea Pig IgG (H&L) F(ab')2 Fragment secondary antibody
0.5 mg / US$ 295
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Guinea Pig IgG (H&L) F(ab')2 Fragment
Product Description
Goat anti Guinea Pig IgG (H&L) F(ab')2 Fragment , Presentation: HRP
Properties
| Presentation | HRP |
| Host | Goat |
| Catalog Number | R1393HRP |
| Quantity | 0.5 mg |
| Price | US$ 295 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1393HRP.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Guinea Pig IgG whole molecule |
| Format | State: Lyophilized F(ab')2 fragments Purification: Immunoaffinity chromatography BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative Label: Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons) Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:40,000 is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
| Storage | Store vial at 4° C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 4°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation) |
