R1394AP Hamster IgG (H&L) F(ab')2 Fragment secondary antibody
0.5 mg / US$ 335
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Hamster IgG (H&L) F(ab')2 Fragment -
Product Description
Rabbit anti Hamster IgG (H&L) F(ab')2 Fragment -
Properties
| Presentation | AP |
| Host | Rabbit |
| Catalog Number | R1394AP |
| Quantity | 0.5 mg |
| Price | US$ 335 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1394AP.pdf |
Datasheet Extract
| F(ab')2 Fragment of Rabbit// anti-Golden Syrian Hamster IgG [H&L] -AP- | |
| Concentration | 0.6 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Golden Syrian Hamster IgG whole molecule |
| Format | State: Lyophilized F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). Reconstitution: Restore with 0.5 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:3,000 - 1:12,000. Western Blot: 1:500 - 1:2,000. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Alkaline Phosphatase, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG. |
| Storage | Store the antibody as an undiluted liquid at 4°C before opening.
DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: One year from despatch. |
| References | 1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
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