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R1394F  Hamster IgG (H&L) F(ab')2 Fragment secondary antibody

0.5 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Hamster IgG (H&L) F(ab')2 Fragment -

Product Description

Rabbit anti Hamster IgG (H&L) F(ab')2 Fragment -, Presentation: FITC

Properties

PresentationFITC
HostRabbit
Catalog NumberR1394F
Quantity0.5 mg
PriceUS$ 315
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1394F.pdf

Datasheet Extract

F(ab')2 Fragment of Rabbit //anti-Golden Syrian Hamster IgG [H&L] -FITC-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenGolden Syrian Hamster IgG whole molecule.
Format
State: Lyophilized F(ab')2 fragments.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Reconstitution: Restore with 0.5 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Fluorescein, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.
StorageStore vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
References1. The and Feltkamp, Immunology 18; 865, 1970.