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R1396AP  Human IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Ms, Rt secondary antibody

0.5 mg / US$ 335
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Human IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Ms, Rt

Product Description

Goat anti Human IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Ms, Rt

Properties

PresentationAP
HostGoat
Catalog NumberR1396AP
Quantity0.5 mg
PriceUS$ 335
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1396AP.pdf

Datasheet Extract

Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenHuman IgG whole molecule.
Format
State: Liquid (sterile filtered) F(ab')2 fragments.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.01 M Tris Chloride, 0.15M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.05% (w/v) sodium azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product.
SpecificityThis product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgG and Human Serum.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.
StorageStore the antibody as an undiluted liquid at 4°C.
DO NOT FREEZE!
Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.