R1396R Human IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Ms, Rt secondary antibody
1 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Human IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Ms, Rt
Product Description
Goat anti Human IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Ms, Rt
Properties
| Presentation | PE |
| Host | Goat |
| Catalog Number | R1396R |
| Quantity | 1 ml |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1396R.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound). |
| Immunogen | Human IgG whole molecule |
| Format | State: Lyophilized F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride; pH 7.2 with 0.05% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free, as stabilizer. Label: R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons). Molarratio: 490 nm, 545 nm and 565 nm / 580 nm Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Recommended dilutions: Flow cytometry: 1:100 - 1:200 IF Mocroscopy: 1:100 - 1:200 |
| Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), or Bovine, Horse, Mouse and Rat Serum Proteins. |
| Storage | Store vial at 4°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
