R1403B Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins secondary antibody
0.5 mg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins
Product Description
Rabbit anti Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins , Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Rabbit |
| Catalog Number | R1403B |
| Quantity | 0.5 mg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1403B.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Mouse IgG whole molecule |
| Format | State: Lyophilized F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molarratio: 10-20 BAC molecules per Rabbit IgG F(ab')2 molecule Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:70,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins. |
| Storage | Store vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
