R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins secondary antibody
1 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins -
Product Description
Rabbit anti Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins -
Properties
| Presentation | PE |
| Host | Rabbit |
| Catalog Number | R1403R |
| Quantity | 1 ml |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1403R.pdf |
Datasheet Extract
| F(ab')2 Fragment of //Rabbit anti-Mouse IgG [H&L] -PE- | |
| Concentration | Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 8.2 at 565 nm for a 0.1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound). |
| Immunogen | Mouse IgG whole molecule. |
| Format | State: Liquid (sterile filtered) F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA; pH 8.0, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer. Label: R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed. Absorptionemission: 488 nm / 575 nm |
| Applications | Suitable for Immunomicroscopy (1:100-1:250) and Flow Cytometry (1:100-1:250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
| Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins. |
| Add. Information | OD566nm/OD280nm: 2.8
|
| Storage | This antibody is stable at 2-8°C as an undiluted liquid.
DO NOT FREEZE! Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. Dilute only prior to immediate use. Shelf life: One year from despatch. |