R1409AP Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins secondary antibody
see all 7 Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins products
0.5 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins -
Product Description
Goat anti Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins -
Properties
| Presentation | AP |
| Host | Goat |
| Catalog Number | R1409AP |
| Quantity | 0.5 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1409AP.pdf |
Datasheet Extract
| F(ab')2 fragment of Goat// anti-Mouse IgG, IgA, IgM [H&L] -AP- | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Mouse IgG, IgA and IgM whole molecules. |
| Format | State: Liquid (sterile filtered) F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protesase free) as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). |
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: ELISA: 1:6,300 Western Blot: 1:200 - 1:1,000 Immunohistochemistry: 1:100 - 1:500 |
| Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. This reagent is suitable for the detection of all mouse immunoglobulin subclasses and chain combinations. |
| Storage | This antibody is stable at 2-8°C as an undiluted liquid.
DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: One year from despatch. |
| References | 1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
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