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R1409B  Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins


see all 7 Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins products
1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins -

Product Description

Goat anti Mouse IgA + IgG + IgM (H&L) F(ab')2 Fragment ads. to Hu serum proteins -, Presentation: Biotin

Properties

PresentationBiotin
HostGoat
Catalog NumberR1409B
Quantity1 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1409B.pdf

Datasheet Extract

F(ab')2 Fragment of Goat //anti-Mouse IgG, IgA, IgM [H&L] -Biotin-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenMouse IgG, IgA and IgM whole molecules.
Format
State: Lyophilized F(ab')2 fragments.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Molarratio: 10-20 BAC molecules per Goat IgG molecule.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1:5,000-1:20,000
Western Blot: 1:500-1:2,000
Immunohistochemistry: 1:500-1:2,000
SpecificityThis product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.
No reaction was observed against Human Serum proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
StorageStore vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.