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R1411TR  Rabbit IgG F(c) F(ab')2 Fragment secondary antibody

1 mg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rabbit IgG F(c) F(ab')2 Fragment

Product Description

Goat anti Rabbit IgG F(c) F(ab')2 Fragment , Presentation: Texas Red

Properties

PresentationTexas Red
HostGoat
Catalog NumberR1411TR
Quantity1 mg
PriceUS$ 375
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1411TR.pdf

Datasheet Extract

Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRabbit IgG F(c) fragment.
Format
State: Lyophilized F(ab')2 fragments.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Texas Red -- Sulfonyl Chloride (TR; Molecular Weight 625 daltons).
Absorptionemission: 596 nm / 620 nm
Molarratio: 1.2 moles Texas Red TM per mole of Goat IgG F(ab')2.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
SpecificityThis product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab')2.
StorageStore vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References1. J.A. Titus, et al. J. Immunol. Methods 50; 193, 1982.