R1414AP Rat IgG F(c) F(ab')2 Fragment ads. to Bov, Eq, Hu secondary antibody
0.5 mg / US$ 395
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Rat IgG F(c) F(ab')2 Fragment ads. to Bov, Eq, Hu
Product Description
Goat anti Rat IgG F(c) F(ab')2 Fragment ads. to Bov, Eq, Hu
Properties
| Presentation | AP |
| Host | Goat |
| Catalog Number | R1414AP |
| Quantity | 0.5 mg |
| Price | US$ 395 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1414AP.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Rat IgG F(c) fragment |
| Format | State: Liquid (sterile filtered) F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). |
| Applications | Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s): This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F(ab')2 or Bovine, Horse, and Human Serum Proteins. |
| Storage | The antibody is stable at 2-8°C as undiluted liquid.
DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use. Shelf life: One year from despatch. |
| References | 1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. |
