R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. secondary antibody
1 ml / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Donkey anti Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. -
Product Description
Donkey anti Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. -
Properties
| Presentation | PE |
| Host | Donkey |
| Catalog Number | R1427R |
| Quantity | 1 ml |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1427R.pdf |
Datasheet Extract
| F(ab')2 Fragment of anti-Rabbit IgG [H&L]-PE | |
| Immunogen | Rabbit IgG whole molecule |
| Format | State: Lyophilized F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride; pH 7.2 with 0.02% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer. Label: R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons).Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound). Absorptionemission: 490 nm, 545 nm and 565 nm / 580 nm Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunomicroscopy (1:100-1:200) and Flow Cytometry (1:100-1:200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
| Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins. |
| Storage | Store the antibody (undiluted) at 2-8°C prior to opening.
DO NOT FREEZE! Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. Dilute only prior to immediate use. Shelf life: One year from despatch. |
