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R1439C5  Rat IgG (H&L) multi-species ads. secondary antibody

1 mg / US$ 445
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rat IgG (H&L) multi-species ads.

Product Description

Goat anti Rat IgG (H&L) multi-species ads. , Presentation: Cy5

Properties

PresentationCy5
HostGoat
Catalog NumberR1439C5
Quantity1 mg
PriceUS$ 445
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1439C5.pdf

Datasheet Extract

Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgG, whole molecule
Format
State: Lyophilized
Purification: Immunoaffinity chromatography
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer

Label: Cy5 (Molecular weight 975 daltons)
Absorptionemission: 650 nm / 667 nm
Molarratio: 10.2 moles Cy5 per mole of Goat IgG
Reconstitution: Restore with 1.0 ml of deionized water or equivalent.
ApplicationsSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Minimum ELISA Endpoint Dilution: 1:10,000.
Actual: 1:110,000. Disposition: PASS.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.
Only minimum cross reactivity was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Sheep and Human Serum Proteins.
Actual cross reactivities:
Bovine 0.4%, Chicken 0.01%, Goat 0.4%, Guinea Pig 0.01%, Hamster 0.01%, Horse 0.4%, Mouse 0.01%, Rabbit 0.8%, Sheep 0.4%, Human 0.01%
StorageStore vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.