R1456B Sheep IgG (H&L) secondary antibody
see all 46 Sheep IgG products
2 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Sheep IgG (H&L) -
Product Description
Rabbit anti Sheep IgG (H&L) -, Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Rabbit |
| Catalog Number | R1456B |
| Quantity | 2 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1456B.pdf |
Datasheet Extract
| Polyclonal antibody to Sheep IgG [H&L] - Biotin | |
| Concentration | 2.0 mg/ml ( by UV absorbance at 280 nm) |
| Immunogen | Sheep IgG whole molecule |
| Format | State: Lyophilized IgG fraction Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% Sodium Azide as preservative Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molarratio: 10-20 BAC molecules per Rabbit IgG molecule Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 is suggested for this product. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG, and Sheep Serum. |
| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (see 'Format'). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Shelf life: one year from despatch. |
| References | Bayer & Wilchek: Methods in Enzymology 184; 138-160, 1990. (Conjugation) |
