R1456HRP Sheep IgG (H&L) secondary antibody
see all 67 Sheep IgG products
2 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Sheep IgG (H&L)
Product Description
Rabbit anti Sheep IgG (H&L) , Presentation: HRP
Properties
| Presentation | HRP |
| Host | Rabbit |
| Catalog Number | R1456HRP |
| Quantity | 2 mg |
| Price | US$ 315 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1456HRP.pdf |
Datasheet Extract
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Sheep IgG whole molecule. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as a stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: Horseradish Peroxidase (HRP) Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Immunoblotting: Western or dot blot (1/1,000-1/5,000 for Western blot).
ELISA (1/100,000). Immunohistochemistry (1/500-1/2,500). Immunoperoxidase electron microscopy as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. |
| Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum. |
| Storage | Store vial at 2-8°C prior to restoration.
Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | 1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation) |
