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R1456T  Sheep IgG (H&L) secondary antibody


see all 67 Sheep IgG products
2 mg / US$ 285
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

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Rabbit anti Sheep IgG (H&L)

Product Description

Rabbit anti Sheep IgG (H&L) , Presentation: TRITC

Properties

PresentationTRITC
HostRabbit
Catalog NumberR1456T
Quantity2 mg
PriceUS$ 285
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1456T.pdf

Datasheet Extract

Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenSheep IgG whole molecule.: 570 nm
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservatives.
Label: Tetramethylrhodamine isothiocyanante (TRITC; Molecular Weight 444 daltons)
Absorptionemission: 550 nm / 570 nm
Molarratio: 3.2 moles TRITC per mole of Rabbit IgG.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
SpecificityThis product was prepared from monospecific antiserum by Immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.
StorageStore vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. J.A. Titus, et al. J. Immunol. Methods 50; 193, 1982.