![<b>Figure 1.</b> Western blot using Affinity Purified
anti-BACH1 antibody shows detection of a band at
~105 kDa (lane 1) corresponding to human BACH1
present in a 293 whole cell lysate (arrowhead). Lane 2
shows that specific band staining is competed out when
the antibody is pre-incubated with the peptide
immunogen prior to reaction. Approximately 35 µg of
lysate was separated on a 4-20% Tris-Glycine gel by
SDS-PAGE and transferred onto nitrocellulose. After
blocking the membrane was probed with the primary
antibody diluted to 1:1,000. Reaction occurred 2 h at
room temperature followed by washes and reaction
with a 1:10,000 dilution of IRDye TM800 conjugated Gt-a-
Rabbit IgG [H&L] MX for 45 min at room
temperature (800 nm channel, green). Molecular
weight estimation was made by comparison to
prestained MW markers in lane M (700 nm channel,
red). IRDye TM800 fluorescence image was captured
using the Odyssey® Infrared Imaging System
developed by LI-COR. IRDye is a trademark of LI-COR,
Inc. Other detection systems will yield similar results.](/media/images/0094707.jpeg)
R1476P Fanconi Anemia Group J Protein antibody
see related secondary antibodiessee all 14 Fanconi Anemia Group J Protein products
0.1 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Fanconi Anemia Group J Protein
Synonyms
BRIP1, BACH1, FANCJ, Protein FACJ, ATP-dependent RNA helicase BRIP1, BRCA1-interacting protein C-terminal helicase 1, BRCA1-interacting protein 1, BRCA1-associated C-terminal helicase 1
Product Description
Rabbit anti Fanconi Anemia Group J Protein , Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | R1476P |
| Swiss Prot. No. | Q9BX63 |
| Quantity | 0.1 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1476P.pdf |
Datasheet Extract
| Alternate names | BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1, BRCA1-associated C-terminal helicase 1 |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Background | BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal doublestrand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein.
|
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 92-104 of isoform 1 of human BACH1 protein. Swiss Num.: Q9BX63 AA Sequence: Protein Sequence: Human BACH1 (isoform 1), 1249 aa, predicted MW 140.8 kDa 1 mssmwseyti ggvkiyfpyk aypsqlammn silrglnskq hcllesptgs gkslallcsa 61 lawqqslsgk padegvseka evqlscccac hskdftnndm nqgtsrhfny pstppserng 121 tsstcqdspe kttlaaklsa kkqasiyrde nddfqvekkr irplettqqi rkrhcfgtev 181 hnldakvdsg ktvklnsple kinsfspqkp pghcsrcccs tkqgnsqess ntikkdhtgk 241 skipkiyfgt rthkqiaqit relrrtaysg vpmtilssrd htcvhpevvg nfnrnekcme 301 lldgkngksc yfyhgvhkis dqhtlqtfqg mckawdieel vslgkklkac pyytareliq 361 dadiifcpyn ylldaqires mdlnlkeqvv ildeahnied caresasysv tevqlrfard 421 eldsmvnnni rkkdheplra vccslinwle anaeylverd yesackiwsg nemlltlhkm 481 gittatfpil qghfsavlqk eekispiygk eearevpvis astqimlkgl fmvldylfrq 541 nsrfaddyki aiqqtyswtn qidisdkngl lvlpknkkrs rqktavhvln fwclnpavaf 601 sdingkvqti vltsgtlspm ksfsselgvt ftiqleanhi iknsqvwvgt igsgpkgrnl 661 catfqntetf efqdevgall lsvcqtvsqg ilcflpsykl leklkerwls tglwhnlelv 721 ktvivepqgg ektnfdellq vyydaikykg ekdgallvav crgkvsegld fsddnaravi 781 tigipfpnvk dlqvelkrqy ndhhsklrgl lpgrqwyeiq ayralnqalg rcirhrndwg 841 alilvddrfr nnpsryisgl skwvrqqiqh hstfesales laefskkhqk vlnvsikdrt 901 niqdnestle vtslkystpp ylleaashls penfvedeak icvqelqcpk iitknsplps 961 siisrkeknd pvfleeagka ekivisrsts ptfnkqtkrv swssfnslgq yftgkipkat 1021 pelgssensa sspprfktek mesktvlpft dkcessnltv ntsfgscpqs etiisslkid 1081 atltrknhse hplcseeald pdielslvse edkqstsnrd feteaedesi yftpelydpe 1141 dtdeekndla etdrgnrlan nsdcilakdl feirtikevd sarevkaedc idtklngilh 1201 ieeskiddid gnvkttwine lelgktheie iknfkpspsk nkgmfpgfk |
| Format | State: Liquid (sterile filtered) purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. |
| Applications | This affinity purified antibody has been tested for use in ELISA (1:10,000-1:44,000) and Western blot (1:500-1:2,000).
Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 105 -140 kDa in size corresponding to isoforms of BACH1 protein by Western blotting in the appropriate cell lysate or extract. |
| Description | AASequence: Human BACH1 (isoform 1), 1249 aa, predicted MW 140.8 kDa 1 mssmwseyti ggvkiyfpyk aypsqlammn silrglnskq hcllesptgs gkslallcsa 61 lawqqslsgk padegvseka evqlscccac hskdftnndm nqgtsrhfny pstppserng 121 tsstcqdspe kttlaaklsa kkqasiyrde nddfqvekkr irplettqqi rkrhcfgtev 181 hnldakvdsg ktvklnsple kinsfspqkp pghcsrcccs tkqgnsqess ntikkdhtgk 241 skipkiyfgt rthkqiaqit relrrtaysg vpmtilssrd htcvhpevvg nfnrnekcme 301 lldgkngksc yfyhgvhkis dqhtlqtfqg mckawdieel vslgkklkac pyytareliq 361 dadiifcpyn ylldaqires mdlnlkeqvv ildeahnied caresasysv tevqlrfard 421 eldsmvnnni rkkdheplra vccslinwle anaeylverd yesackiwsg nemlltlhkm 481 gittatfpil qghfsavlqk eekispiygk eearevpvis astqimlkgl fmvldylfrq 541 nsrfaddyki aiqqtyswtn qidisdkngl lvlpknkkrs rqktavhvln fwclnpavaf 601 sdingkvqti vltsgtlspm ksfsselgvt ftiqleanhi iknsqvwvgt igsgpkgrnl 661 catfqntetf efqdevgall lsvcqtvsqg ilcflpsykl leklkerwls tglwhnlelv 721 ktvivepqgg ektnfdellq vyydaikykg ekdgallvav crgkvsegld fsddnaravi 781 tigipfpnvk dlqvelkrqy ndhhsklrgl lpgrqwyeiq ayralnqalg rcirhrndwg 841 alilvddrfr nnpsryisgl skwvrqqiqh hstfesales laefskkhqk vlnvsikdrt 901 niqdnestle vtslkystpp ylleaashls penfvedeak icvqelqcpk iitknsplps 961 siisrkeknd pvfleeagka ekivisrsts ptfnkqtkrv swssfnslgq yftgkipkat 1021 pelgssensa sspprfktek mesktvlpft dkcessnltv ntsfgscpqs etiisslkid 1081 atltrknhse hplcseeald pdielslvse edkqstsnrd feteaedesi yftpelydpe 1141 dtdeekndla etdrgnrlan nsdcilakdl feirtikevd sarevkaedc idtklngilh 1201 ieeskiddid gnvkttwine lelgktheie iknfkpspsk nkgmfpgfk Background: BACH1 (also known as BRCA1 interacting protein C-terminal helicase 1, BRCA1-interacting protein 1 and BRCA1-associated C-terminal helicase 1) is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal doublestrand break repair function of breast cancer, type 1 (BRCA1). The BACH1 gene may be a target of germline cancer-inducing mutations. BACH1 is localized within the nucleus and functions as a DNA-dependent ATPase and 5' to 3' DNA helicase. Two isoforms have been identified for this protein. |
| Specificity | This affinity purified antibody is directed against human BACH1 protein. The product was affinity purified from monospecific antiserum by Immunoaffinity purification.
A BLAST analysis was used to suggest cross reactivity with BACH1 protein from human (100% homology) and chimpanzee (92% homology). Expect reactivity with isoform 1 and isoform 2 of BACH1. Reactivity against BACH1 homologues from rat and mouse is not expected. Reactivity against homologues from other sources is not known. |
| Storage | Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below.
Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Gupta,R., Sharma,S., Sommers,J.A., Jin,Z., Cantor,S.B. and Brosh,R.M. Jr. (2005) Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer. J. Biol. Chem. 280 (27), 25450-25460.
2. Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E., Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101 (33), 12130-12135. 3. Clapperton,J.A., Manke,I.A., Lowery,D.M., Ho,T., Haire,L.F., Yaffe,M.B. and Smerdon,S.J. (2004) Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer. Mol. Cell 11 (6), 512-518. 4. Ohira,M., Seki,N., Nagase,T., Ishikawa,K., Nomura,N. and Ohara,O. (1998) Characterization of a human homolog (BACH1) of the mouse Bach1 gene encoding a BTB-basic leucine zipper transcription factor and its mapping to chromosome 21q22.1. Genomics 47 (2), 300-306. 5.Oyake,T., Itoh,K., Motohashi,H., Hayashi,N., Hoshino,H., Nishizawa,M., Yamamoto,M. and Igarashi,K. (1996) Bach proteins belong to a novel family of BTB-basic leucine zipper transcription factors that interact with MafK and regulate transcription through the NF-E2 site. Mol. Cell. Biol. 16 (11), 6083-6095. |
| Pictures | Figure 1. Western blot using Affinity Purified
anti-BACH1 antibody shows detection of a band at ~105 kDa (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye TM800 conjugated Gt-a- Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye TM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1476P Fanconi Anemia Group J Protein'.
