R1505 c-myc (C-term.) antibody
see related secondary antibodiessee all 136 c-myc products
0.1 ml / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti c-myc (C-term.)
Product Description
Rabbit anti c-myc (C-term.) , Presentation: Serum. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | R1505 |
| Quantity | 0.1 ml |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1505.pdf |
Datasheet Extract
| Concentration | 85 mg/ml (by Refractometry) |
| Background | c-myc is involved in the control of cell proliferation and differentiation at the transition from S --> G1 phase for a normal cell. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (k light chain); or 22 (l light chain) is described in Burkitt's lymphoma and other B-cell lympho-proliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms. Abnormal expression can take many forms including transduction, insertional activation, translocation, and amplification. |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with c-myc peptide corresponding to amino acids KHKLEQLRNSGA which map to the last 12 amino acids of the mouse protein at the carboxy terminus of the protein. |
| Format | State: Liquid (sterile filtered) purified Ig fraction. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. |
| Applications | This antibody reacts with human and mouse c-myc protein, which is a nuclear protein with a molecular weight of approximately 66 kDa.
This antiserum detects c-myc by Immunoblotting assays in both overexpressed and endogenous systems. Reactivity in other immunoassays has not yet been fully determined although perinuclear and cytoplasmic staining has been reported. The recommended positive controls are SKBR-3 cells (adenocarcinoma, breast, malignant pleural effusion, human). This product was assayed by Immunoblot and was found to be reactive at a 1:200-1:1000 dilution. Detection occurs using HRP Donkey anti-Rabbit IgG and ECL. |
| Specificity | This antiserum is directed against human c-myc and is useful in determining its presence in various assays.
Anti-c-myc is highly specific for c-myc and shows no cross-reaction with N-myc or L-myc proteins. |
| Storage | Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below.
Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Bartova, E. et al. (2000) Nuclear topography of the c-myc gene in human leukemic cells Gene 244:1-11. |
| Pictures | Figure 1. Anti-c-myc is shown to detect c-myc in infected cells (lanes 2-4) showing a 66 kDa band. No band specific staining is observed in uninfected cells (lane 1). Detection occurs
using a 1:250 dilution of antibody followed by 1:2,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1505 c-myc (C-term.)'.
