R1539P Aurora-B / STK12 / ARK2
see related secondary antibodiessee all 14 Aurora-B / STK12 / ARK2 products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Aurora-B / STK12 / ARK2 -
Synonyms
Aurora kinase B Aurora B STK-1 AIK2 AIM1 AURKB FocusOn123 Serine/Threonine-Protein Kinase 12, Aurora- and Ipl1-like Midbody-Associated Protein 1, Aurora/IPL1-Related Kinase 2
Product Description
Rabbit anti Aurora-B / STK12 / ARK2 -, Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | R1539P |
| Swiss Prot. No. | Q96GD4 |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1539P.pdf |
Datasheet Extract
| Polyclonal Antibody to Human Aurora Kinase B | |
| Alternate names | Aurora- and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, Aurora-B |
| Concentration | 0.45 mg/ml (by UV absorbance at 280 nm) |
| Background | Aurora Kinase B (also known as Aurora- and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 227-238 of Human Aurora Kinase B protein. Swiss Num.: Q96GD4 AA Sequence: Human Aurora B Kinase, 344 aa, predicted MW 39.3kDa 1 maqkensypw pygrqtapsg lstlpqrvlr kepvtpsalv lmsrsnvqpt aapgqkvmen 61 ssgtpdiltr hftiddfeig rplgkgkfgn vylarekksh fivalkvlfk sqiekegveh 121 qlrreieiqa hlhhpnilrl ynyfydrrri ylileyaprg elykelqksc tfdeqrtati 181 meeladalmy chgkkvihrd ikpenlllgl kgelkiadfg wsvhapslrr ktmcgtldyl 241 ppemiegrmh nekvdlwcig vlcyellvgn ppfesashne tyrrivkvdl kfpasvptga 301 qdliskllrh npserlplaq vsahpwvran srrvlppsal qsva |
| Format | State: Liquid (sterile filtered) purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. |
| Applications | This affinity purified antibody has been tested for use in ELISA (1:5,000-1:20,000) and by Western blot (1:500-1:2,000). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLa cell lysate can be used as a positive control. |
| Description | AASequence: Human Aurora B Kinase, 344 aa, predicted MW 39.3kDa 1 maqkensypw pygrqtapsg lstlpqrvlr kepvtpsalv lmsrsnvqpt aapgqkvmen 61 ssgtpdiltr hftiddfeig rplgkgkfgn vylarekksh fivalkvlfk sqiekegveh 121 qlrreieiqa hlhhpnilrl ynyfydrrri ylileyaprg elykelqksc tfdeqrtati 181 meeladalmy chgkkvihrd ikpenlllgl kgelkiadfg wsvhapslrr ktmcgtldyl 241 ppemiegrmh nekvdlwcig vlcyellvgn ppfesashne tyrrivkvdl kfpasvptga 301 qdliskllrh npserlplaq vsahpwvran srrvlppsal qsva |
| Specificity | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Aurora Kinase B protein. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known. |
| Storage | Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Shindo M., et al. (1998) cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2. Biochem. Biophys. Res. Commun. 244:285-292.
2. Gruneberg,U., Neef,R., Honda,R., Nigg,E.A. and Barr,F.A. (2004) Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2. J. Cell Biol. 166 (2), 167-172. 3. Kimura,M., Uchida,C., Takano,Y., Kitagawa,M. and Okano,Y. (2004) Cell cycle-dependent regulation of the human aurora B promoter. Biochem. Biophys. Res. Commun. 316 (3), 930-936. 4. Yasui,Y., Urano,T., Kawajiri,A., Nagata,K., Tatsuka,M., Saya,H., Furukawa,K., Takahashi,T., Izawa,I. and Inagaki,M. (2004) Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis. J. Biol. Chem. 279 (13), 12997-13003. 5. Araki,K., Nozaki,K., Ueba,T., Tatsuka,M. and Hashimoto,N. (2004) High expression of Aurora-B/Aurora and Ipll-like midbody-associated protein (AIM-1) in astrocytomas. J. Neurooncol. 67 (1-2), 53-64. 6. Wheatley,S.P., Henzing,A.J., Dodson,H., Khaled,W. and Earnshaw,W.C. (2004) Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo. J. Biol. Chem. 279 (7), 5655-5660. 7. Honda,R., Korner,R. and Nigg,E.A. (2003) Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis. Mol. Biol. Cell 14 (8), 3325-3341. 8. Bolton,M.A. et al. (2002) Aurora B Kinase Exists in a Complex with Survivin and INCENP and Its Kinase Activity Is Stimulated by Survivin Binding and Phosphorylation Mol. Biol. Cell 13 (9), 3064-3077. |
| Pictures | Figure shows a model of Aurora B, INCENP, and
Survivin interactions and regulation. Survivin binds the N terminus of INCENP, whereas the N terminus of Aurora B binds the C terminus of INCENP. See Bolton et al. (2002). |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 Euro | |||||
| Acris Antibodies GmbH | |||||||
Click here to see all secondary antibodies for this product.