![Figure. Western blot using Affinity Purified anti-MAD2L1 antibody shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates using the 800 nm channel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively) which completely blocks antibody staining. ~ 35 µg of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeTM 800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (lane 3). IRDye TM 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](/media/images/0094577.jpeg)
R1540P MAD2L1 antibody
see related secondary antibodiessee all 22 MAD2L1 products
0.1 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti MAD2L1
Product Description
Rabbit anti MAD2L1 , Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E )
Properties
| Applications | Enzyme Immunoassay ( E ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | R1540P |
| Swiss Prot. No. | Q13257 |
| Quantity | 0.1 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1540P.pdf |
Datasheet Extract
| Alternate names | Mitotic Spindle Assembly Checkpoint Protein, MAD2A, MAD2-like 1, HsMAD2 |
| Concentration | 1.19 mg/ml (by UV absorbance at 280 nm) |
| Background | MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. This protein has a nuclear localization. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 3-13 of Human MAD2L1 protein. Swiss Num.: Q13257 AA Sequence: Human MAD2L1, 205 amino acids, predicated MW 23.5 kDa. 1 malqlsreqg itlrgsaeiv aeffsfgins ilyqrgiyps etftrvqkyg ltllvttdle 61 likylnnvve qlkdwlykcs vqklvvvisn iesgevlerw qfdiecdkta kddsapreks 121 qkaiqdeirs virqitatvt flpllevscs fdlliytdkd lvvpekwees gpqfitnsee 181 vrlrsfttti hkvnsmvayk ipvnd |
| Format | State: Liquid (sterile filtered) purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. |
| Applications | This affinity purified antibody has been tested for use in ELISA (1:2,000-1:10,000) and by Western blot (1:500-1:2,000). Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full length protein by western blotting in the appropriate cell lysate or extract. |
| Description | AASequence: Human MAD2L1, 205 amino acids, predicated MW 23.5 kDa. 1 malqlsreqg itlrgsaeiv aeffsfgins ilyqrgiyps etftrvqkyg ltllvttdle 61 likylnnvve qlkdwlykcs vqklvvvisn iesgevlerw qfdiecdkta kddsapreks 121 qkaiqdeirs virqitatvt flpllevscs fdlliytdkd lvvpekwees gpqfitnsee 181 vrlrsfttti hkvnsmvayk ipvnd |
| Specificity | This affinity purified antibody is directed against human MAD2L1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, dog, macaque, chimpanzee and gecko based on 100% homology for the immunogen sequence. Cross reactivity with MAD2L1 may occur from mouse and chicken sources, as only a two amino acid residue change is found within the immunogen sequence (90% positive by BLAST). Cross reactivity with MAD2L1 homologues from other sources has not been determined. |
| Storage | Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Wang,R.H., Yu,H. and Deng,C.X. (2004) A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint. Proc. Natl. Acad. Sci. U.S.A. 101 (49), 17108-17113.
2. Wu,C.W., Chi,C.W. and Huang,T.S. (2004) Elevated level of spindle checkprotein MAD2 correlates with cellular mitotic arrest, but not with aneuploidy and clinicopathological characteristics in gastric cancer. World J. Gastroenterol. 10 (22), 3240-3244. 3. Li,G.Q. and Zhang,H.F. (2004) Mad2 and p27 expression profiles in colorectal cancer and its clinical significance. World J. Gastroenterol. 10 (21), 3218-3220. 4. Sze,K.M., Ching,Y.P., Jin,D.Y. and Ng,I.O. (2004) Association of MAD2 expression with mitotic checkpoint competence in hepatoma cells. J. Biomed. Sci. 11 (6), 920-927. 5. Hernando,E., Nahle,Z., Juan,G., Diaz-Rodriguez,E., Alaminos,M., Hemann,M., Michel,L., Mittal,V., Gerald,W., Benezra,R., Lowe,S.W. and Cordon-Cardo,C. (2004) Rb inactivation promotes genomic instability by uncoupling cell cycle progression from mitotic control. Nature 430 (7001), 797-802. |
| Pictures | Figure. Western blot using Affinity Purified anti-MAD2L1 antibody shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates using the 800 nm channel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively) which completely blocks antibody staining. ~ 35 µg of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeTM 800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (lane 3). IRDye TM 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1540P MAD2L1'.
