R1541P MAD2L2 antibody
see related secondary antibodiessee all 6 MAD2L2 products
0.1 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti MAD2L2
Product Description
Rabbit anti MAD2L2 , Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E )
Properties
| Applications | Enzyme Immunoassay ( E ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | R1541P |
| Quantity | 0.1 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1541P.pdf |
Datasheet Extract
| Alternate names | MAD2B, REV7, Mitotic arrest deficient-like 2 protein. |
| Concentration | 0.81 mg/ml (by UV absorbance at 280 nm) |
| Background | MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1. |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2. Swiss Num.: Q9UI95 (Human) AA Sequence: Protein sequence: Human sequence, 211 amino acids, predicated MW 24.3 kDa. 1 MTTLTRQDLN FGQVVADVLC EFLEVAVHLI LYVREVYPVG IFQKRKKYNV PVQMSCHPEL 61 NQYIQDTLHC VKPLLEKNDV EKVVVVILDK EHRPVEKFVF EITQPPLLSI SSDSLLSHVE 121 QLLRAFILKI SVCDAVLDHN PPGCTFTVLV HTREAATRNM EKIQVIKDFP WILADEQDVH 181 MHDPRLIPLK TMTSDILKMQ LYVEERAHKG S |
| Format | State: Liquid (sterile filtered) purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. |
| Applications | This affinity purified antibody has been tested for use in ELISA (1:7,500-1:30,000) against the immunizing peptide and by Western blotting (1:500-1:2,000) against HeLa whole cell lysates and nuclear extracts. Reactivity in other immunoassays is unknown. Antibody activity can be blocked using a control peptide. |
| Description | AASequence: Human sequence, 211 amino acids, predicated MW 24.3 kDa. 1 MTTLTRQDLN FGQVVADVLC EFLEVAVHLI LYVREVYPVG IFQKRKKYNV PVQMSCHPEL 61 NQYIQDTLHC VKPLLEKNDV EKVVVVILDK EHRPVEKFVF EITQPPLLSI SSDSLLSHVE 121 QLLRAFILKI SVCDAVLDHN PPGCTFTVLV HTREAATRNM EKIQVIKDFP WILADEQDVH 181 MHDPRLIPLK TMTSDILKMQ LYVEERAHKG S |
| Specificity | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. |
| Storage | Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Chen J, Fang G. (2001) MAD2B is an inhibitor of the anaphase-promoting complex. Genes Dev. 15(14):1765-70.
2. Nelson K.K., Schlondorff J., Blobel C.P. (1999) Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2-beta. Biochem. J. 343:673-680. 3. Cahill D.P., da Costa L.T., Carson-Walter E.B., Kinzler K.W., Vogelstein B., Lengauer C. (1999) Characterization of MAD2B and other mitotic spindle checkpoint genes. Genomics 58:181-187. 4. Murakumo Y., Roth T., Ishii H., Rasio D., Numata S., Croce C.M., Fishel R. (2000) A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2. J. Biol. Chem. 275:4391-4397. |
| Pictures | Figure 1. Immunoblotting. Affinity Purified Rabbit anti- MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1541P MAD2L2'.
