R1591P Macrophage Migration Inhibitory Factor (MIF) antibody
see related secondary antibodiessee all 8 Macrophage Migration Inhibitory Factor products
0.5 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Chicken anti Macrophage Migration Inhibitory Factor (MIF)
Product Description
Chicken anti Macrophage Migration Inhibitory Factor (MIF) , Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Chicken |
| Catalog Number | R1591P |
| Quantity | 0.5 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1591P.pdf |
Datasheet Extract
| Concentration | 5.0 mg/ml (by UV absorbance at 280 nm) |
| Background | Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/ml to 10 ng/ml of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18-kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.
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| Immunogen | This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events. AA Sequence: P-M-F-I-V-N-T-N-V-P-R-A-S-V-P-D-G-F-L-S-E-L-T-Q-Q-L-A-Q-A-T-G |
| Format | State: Lyophilized purified Ig fraction. Purification: Multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. Reconstitution: Restore with 0.1 ml of deionized water (or equivalent). |
| Applications | This IgY fraction antibody of anti-Human Macrophage
Migration Inhibitory Factor (MIF) has been tested for use in ELISA and Immunoblotting. Although not tested, this antibody may also be useful for Neutralization assays, Immunohistochemistry and Flow Cytometry. The antibody recognizes 12,300 MW mature human MIF. The MIF gene encodes a protein of 115 aa. The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown. Immunoblot: Using IRDYE800 conjugated Goat-a-Chicken IgG [H&L] MX10. A working dilution range of 1:500-1:1,000 is suggested for this application to detect human MIF from supernatants or lysates of 2 x 10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. This product has been assayed by ELISA against human MIF using HRP Conjugated Goat-a-Chicken IgG [H&L] MX10 and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1,000-1:2,000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. |
| Specificity | This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F( c) domain of the chicken IgG molecule may interact with cells non-specifically. Endotoxin Content: < 10 pg/µl by LAL method. |
| Storage | Store vial at 2-8°C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Weiser WY et al. (1989). Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proc Natl Acad Sci U S A 86:7522-6.
2. Bernhagen J et al. (1993). MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia. Nature 365:756-9. 3. Calandra, T,. et al. (1995). MIF as a glucocorticoid-induced modulator of cytokine production. Nature 377, 68-71. 4. Mitchell R et al. (1995). Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF). J Immunol 154:3863-70. 5. Rosengren, E., et al, (1996). The immunoregulatory mediator macrophage migration inhibitory factor (MIF) catalyzes a tautomerization reaction. Mol. Med. 2, 143-149. 6. Claret, F. X., et al. (1996). A new group of conserved coactivators that increase the specificity of AP-1 transcription factors. Nature 383, 453-457. 7. Bucala R (1998). Neuroimmunomodulation by macrophage migration inhibitory factor (MIF). Ann N Y Acad Sci 840:74-82. 8. Bernhagen, J., et. al., (1998). Regulation of the immune response by macrophage migration inhibitory factor: biological and structural features. J. Mol. Med. 76, 151-161. 9. Kleemann, R., et al. (1998). Disulfide analysis reveals a role for macrophage migration inhibitory factor (MIF) as a thiol-protein oxidoreductase. J. Mol. Biol. 280, 85-102. 10. Nishihira J, Koyama Y, Mizue Y. (1998) Identification of macrophage migration inhibitory factor (MIF) in human vascular endothelial cells and its induction by lipopolysaccharide. Cytokine 10(3):199-205. 11. Leech M, et al. (1999) Macrophage migration inhibitory factor in rheumatoid arthritis: evidence of proinflammatory function and regulation by glucocorticoids. Arthritis Rheum.42(8):1601-8. 12. Kleemann, R., et al. (2000). Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1. Nature 408, 211-216. 13. Onodera, S., et al. (2000) Macrophage Migration Inhibitory Factor (MIF) up-regulates expression of matrix metalloproteinases in synovial fibroblasts of rheumatoid arthritis. J. Biol. Chem. 275, 444-450. |
11 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1300B | Chicken IgG (H&L) | ||||||
| Rabbit | Biotin | 1.5 mg / US$ 325 | |||||
| R1300F | Chicken IgG (H&L) | ||||||
| Rabbit | FITC | 1.5 mg / US$ 295 | |||||
| R1300T | Chicken IgG (H&L) | ||||||
| Rabbit | TRITC | 1.5 mg / US$ 305 | |||||
| R1300TR | Chicken IgG (H&L) | ||||||
| Rabbit | Texas Red | 1.5 mg / US$ 335 | |||||
| R1300HRP | Chicken IgG (H&L) | ||||||
| Rabbit | HRP | 1.5 mg / US$ 315 | |||||
| R1300AP | Chicken IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 355 | |||||
| R1434C2 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1434C3 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1434C35 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1434C5 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1434C55 | Chicken IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
Click here to see all secondary antibodies for 'R1591P Macrophage Migration Inhibitory Factor (MIF)'.
