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R1614HRP  Rat IgG (H&L) secondary antibody


see all 196 Rat IgG products
2 mg / US$ 295
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti Rat IgG (H&L)

Product Description

Goat anti Rat IgG (H&L) , Presentation: HRP

Properties

PresentationHRP
HostGoat
Catalog NumberR1614HRP
Quantity2 mg
PriceUS$ 295
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1614HRP.pdf

Datasheet Extract

Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenRat IgG whole molecule

Format
State: Lyophilized Ig fraction
Purification: Immunoaffinity chromatography
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as a stabilizer and 0.01% (w/v) Gentamicin Sulfate as a preservative
Label: Horseradish Peroxidase (HRP)
Reconstitution: Restore with 1.0 ml of deionized water or equivalent.
ApplicationsImmunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions:
Western blot 1:1,000 - 1:3,500
ELISA 1:6,500 - 1:32,000
Immunohistochemistry 1:500 - 1:2,500
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum.
StorageStore vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.

ReferencesFarr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)