SM051B CD106 / VCAM1 antibody
see related secondary antibodiessee all 59 CD106 / VCAM1 products
0.1 mg / US$ 335
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti CD106 / VCAM1 M/K-2
Synonyms
VCAM1, VCAM-1, L1CAM, V-CAM 1, INCAM-100, CD106, Vascular cell adhesion protein 1,
Product Description
Rat anti CD106 / VCAM1 M/K-2, Presentation: Biotin. Product is tested for Flow cytometry ( F )
Properties
| Applications | Flow cytometry ( F ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Biotin |
| Host | Rat |
| Isotype | IgG1 |
| Clone | M/K-2 |
| Catalog Number | SM051B |
| Swiss Prot. No. | P29533 |
| Quantity | 0.1 mg |
| Price | US$ 335 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM051B.pdf |
Datasheet Extract
| Alternate names | VCAM-1 |
| Concentration | 0.1 mg/ml |
| Background | VCAM-1 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin). |
| Immunogen | Swiss Num.: P29533 |
| Format | State: Liquid Ig fraction BufferSystem: PBS, containing 0.09% sodium azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: Biotin |
| Applications | Flow cytometry.
The clone is reported to work with frozen sections. |
| Specificity | This anti-mouse CD106 (VCAM-1) monoclonal antibody recognizes an antigen that is constitutively expressed on bone marrow stromal cells and myeloid cells, and its expression on endothelial cells is upregulated by inflammatory cytokines and in certain pathologic conditions. Species: Mouse. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Kinashi, T. and T.A. Springer. 1994. Adhesion molecules in hematopoetic cells. Blood Cells. 20: 25-44.
|
| Protocols | Flow Cytometry Analysis:
Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1.0 µg* of SM051B per 1 x 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-PE) at a dilution suggested by the supplier. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). * Appropriate control samples should always be included in any labelling studies |
| Pictures | Bone marrow population: 0.25 µg of mouse CD106 antibody on C57BL/6 strain mouse bone marrow cells |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 335 | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM051B CD106 / VCAM1'.
