SM1303F B-Cells (FMC7 Antigen) antibody
see related secondary antibodiessee all 41 B-Cells products
100 Tests / US$ 635
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti B-Cells (FMC7 Antigen) FMC7
Synonyms
B Cells
Product Description
Mouse anti B-Cells (FMC7 Antigen) FMC7, Presentation: FITC. Product is tested for Flow cytometry ( F )
Properties
| Applications | Flow cytometry ( F ) |
| Reactivity | Human ( Hu ) |
| Presentation | FITC |
| Host | Mouse |
| Isotype | IgM |
| Clone | FMC7 |
| Catalog Number | SM1303F |
| Quantity | 100 Tests |
| Price | US$ 635 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM1303F.pdf |
Datasheet Extract
| Monoclonal Antibody to Human B-Cells // FITC | |
| Concentration | 0.1 mg/ml |
| Background | The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. |
| Immunogen | HRIK cells - Human B-Lymphoblastoid line. |
| Format | State: Liquid purified IgM Purification: Gel filtration BufferSystem: 0.1M Tris-HCL, 0.5ml NaCl, 10mM glycine pH8.0 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: Fluorescein Isothiocyanate Isomer 1 (FITC) |
| Applications | Flow cytometry. |
| Specificity | This antibody recognises a glycoprotein antigen of 105kD expressed by B lymphocytes. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C.
DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch. |
| References | 1. Brooks D.A., et al. (1981) Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes. J. Immunol.
126: 1373. 2. Catovsky D., et al. (1981) Heterogeneity of B cell leukaemias demonstrated by the monoclonal antibody FMC7. Br. J. Haematol. 49: 137. 3. Zola H., et al. (1984) The human B cell lineage studied with monoclonal antibodies. In Leucocyte Typing Ed.A. Bernard, Springer Verlag. p363-371. 4. Zola H., et al. (1984) The antigen of mature human B cells detected by the monoclonal antibody FMC7; studies on the nature of the antigen and its expression. J. Immunol. 133: 321-326. 5. Bloem A.C., et al. (1988) Functional properties of human B cell populations defined by monoclonal antibodies HBA and FMC7. J. Immunol. 140: 768-773. 6. Zola H., et al. (1987) Markers of differentiated B cell leukemia: CD22 antibodies and FMC7 react with different molecules. Dis. Markers 5: 227. 7. Melo I.V., et al. (1988) The different diagnosis between chronic lymphocytic leukemia and other B-cell lymphoproliferative disorders: morphological and immunological studies. In: Chronic Lymphocytic Leukemia (eds. A. Polliack and D. Catovsky) p85. Harwood acad. Publ. London. 8. Ferro, L.M. and Zola H. (1990) Modulation of expression of the antigen identified by FMC7 upon human Blymphocyte activation: evidence for difference between activation in vivo and in vitro. Immunology. 69: 373-378. 9. Collins R.J., et al. (1992) Malignant lymphoma: reactive with the monoclonal antibody FMC7. Pathology 15: 350. 10. Serke, S. et al. (2001) Monoclonal antibody FMC7 detects a conformational epitope on the CD20 molecule:Evidence from phenotyping after Rituxan therapy and transfectant cell analyses. Cytometry (Comm. Clin. Cytometry) 46:98-104 |
| Pictures | Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN BCELLS (FMC7): FITC (SM1303F). |
10 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1349B | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 355 | |||||
| R1349F | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 335 | |||||
| R1349T | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 335 | |||||
| R1349TR | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 355 | |||||
| R1349HRP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 365 | |||||
| R1349AP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | AP | 1 mg / US$ 385 | |||||
| SP1018F | Mouse IgM mu chain | ||||||
| Goat | FITC | Ms | 1 mg / US$ 375 | ||||
| SP1018R | Mouse IgM mu chain | ||||||
| Goat | PE | Ms | 0.1 mg / US$ 485 | ||||
| SP1018HRP | Mouse IgM mu chain | ||||||
| Goat | HRP | Ms | 1 mg / US$ 385 | ||||
| SP1018AP | Mouse IgM mu chain | ||||||
| Goat | AP | Ms | 0.8 mg / US$ 425 | ||||
Click here to see all secondary antibodies for 'SM1303F B-Cells (FMC7 Antigen)'.
