SM1863PS c-myc Epitope Tag (EQKLISEEDL) antibody
see related secondary antibodiessee all 33 c-myc Epitope Tag products
20 µg / US$ 275
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti c-myc Epitope Tag (EQKLISEEDL) 9E10
Product Description
Mouse anti c-myc Epitope Tag (EQKLISEEDL) 9E10, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 9E10 |
| Catalog Number | SM1863PS |
| Quantity | 20 µg |
| Price | US$ 275 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM1863PS.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | p62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. |
| Immunogen | Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line. |
| Format | State: Liquid purified IgG Purification: Affinity chromatography on Protein G BufferSystem: PBS, pH7.4 containing 0.09% Sodium Azide |
| Applications | ELISA: 1/100 - 1/500.
Western Blot: 1/100 - 1/500; For use under non-reducing conditions. Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 1x10e6 cells in 100µl. Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin sections prior to staining; This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. |
| Specificity | This antibody detects the 62kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-myc tag. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. Proto-oncogene product. Mol. Cell. Biol. 5: 3610 -3616.
2. Spandidos, D.A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res. 7: 1299 -1304. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM1863PS c-myc Epitope Tag (EQKLISEEDL)'.
