SM2011P Macrophages antibody
see related secondary antibodiessee all 58 Macrophages products
0.2 mg / US$ 535
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Macrophages MAC387
Synonyms
Macrophage marker,
Product Description
Mouse anti Macrophages MAC387, Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ), Paraffin Sections ( P ) |
| Reactivity | Bovine ( Bov ), Canine ( Can ), Equine ( Eq ), Feline ( Fe ), Genua Pig ( GP ), Human ( Hu ), Monkey ( Mky ), Por ( Por ), Rabbit ( Rb ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | MAC387 |
| Catalog Number | SM2011P |
| Quantity | 0.2 mg |
| Price | US$ 535 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM2011P.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Immunogen | Human monocytes |
| Format | State: Liquid purified Ig fraction. Purification: Protein G affinity chromatography. BufferSystem: PBS with 0.09% Sodium Azide as preservative. |
| Applications | Immunohistochemistry on frozen sections (1/100-1/200) and paraffin embedded sections (1/100-1/200, requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment prior to staining, see also Ref.2). Recommended positive control tissue: Human Spleen.
Flow Cytometry: Use at 1/50-1/100 (membrane permeabilisation required, see also Ref.1). |
| Specificity | This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. Species: Human, Horse, Cynomolgus Monkey, Rhesus Monkey, Bovine, Baboon, Rabbit, Dog, Cat, Pig, Pig and Rat. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Brandtzaeg, P., et al (1988). MAC387 antibody and detection of formalin resistant myelomonocytic L1 antigen J. Clin. Path. 41: 963-970.
2. Brandtzaeg, P. et al (1992). The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathol. 21: 191-196. 3. Flavell, D.J., Jones, D.B., Wright, D.H. (1987). Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J. Histochem. Cytochem. 35: 1217-1226. 4. Gutierrez, M. et al. (1999). The detection of CD2+, CD4+, CD8+ and WC1+, Tlymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 5. Ramsay, A. D. et al. (1991). Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies. Journal of Pathology. 164: 321 - 328. 6. Christgau, M. et al. (1998). Characterization of Immunocompetent cells in the diseased canine periodontium. Journal of Histochemistry & Cytochemistry. 46 (12): 1443 - 1454. 7. Perez, J. et al. (1999). Immunohistochemical study of the inflammatory infiltrate assocated with equine squamous cell carcinoma. J. Comp. Path. 121: 385 - 397. 8. Obert, L. et al. (2002). Early pathogenesis of transmucosal Feline Immunodeficiency Virus infection. J. Virol. 76 (12): 6311 - 6322. 9. Malik, N. et al. (1998). Apoptosis and Cell proliferation after porcine coronary angioplasty. Circulation. 98: 1657 - 1665. 10. Bagavant, H. et al. (2002). Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis)). Am. J. Pathol. 160: 141 - 149. |
| Pictures | Figure 1. Macrophages stained with SM2011 using enhanced DAB in experimental allergic marmoset brain. Mag. X 400. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM2011P Macrophages'.
