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Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell
lysate (2) probed with RAT ANTI CCT THETA (SM2017P) followed by F(ab')2 RABBIT ANTI RAT IgG:HRP (SP1021HRP).

 

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SM2017P  CCT Theta / TCP1 Theta antibody

see related secondary antibodies
see all 4 CCT Theta / TCP1 Theta products
0.1 mg / US$ 535
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rat anti CCT Theta / TCP1 Theta PK/13/72/8k

Synonyms

CCT8, TCP-1-theta, CCT-theta, TCPQ, KIAA0002, NY-REN-15, , chaperonin containing TCP1 subunit 8 (theta), T-complex protein 1 subunit theta, Renal carcinoma antigen NY-REN-15

Product review
Please read our product review about CCT Theta / TCP1 Theta : Antibodies to CCT.

Product Description

Rat anti CCT Theta / TCP1 Theta PK/13/72/8k, Presentation: Aff - Purified. Product is tested for Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )

Properties

ApplicationsImmunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
ReactivityBovine ( Bov ), Human ( Hu ), Mouse ( Ms ), Rabbit ( Rb )
PresentationAff - Purified
HostRat
IsotypeIgG2a
ClonePK/13/72/8k
Catalog NumberSM2017P
Swiss Prot. No.P50990
Quantity0.1 mg
PriceUS$ 535
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM2017P.pdf

Datasheet Extract

Monoclonal Antibody to CCT Theta
Concentration1.0 mg/ml
BackgroundThe intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.
Immunogen
Swiss Num.: P50990
Format
State: Liquid purified IgG
Purification: Affinity chromatography on Protein G
BufferSystem: PBS containing 0.09% Sodium Azide
ApplicationsImmunoprecipitation.
Western Blot: 1/250 - 1/500; detects a band of approximately 60kD in lysates of heat shocked Hela cells.
SpecificityThis antibody recognises the theta polypeptide of the CCT chaperonin molecule complex.
Species: Bovine, Rabbit, Human, Mouse.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte twodimensional protein database: further characterisation of antibodies to individual subunits. Electrophoresis. 17: 1720-1727.
2. Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations. EMBO J. 19: 5971-5979.
3. Hynes, G.M. Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. J Biol Chem. 275: 18985-18994.
PicturesWestern blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell
lysate (2) probed with RAT ANTI CCT THETA (SM2017P) followed by F(ab')2 RABBIT ANTI RAT IgG:HRP (SP1021HRP).

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1371B Rat IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 295
   
R1371F Rat IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1371T Rat IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1371TR Rat IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1371HRP Rat IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1371AP Rat IgG (H&L)  
  Rabbit AP   1 mg / US$ 335
   
R1439C2 Rat IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1439C3 Rat IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1439C35 Rat IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1439C5 Rat IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1439C55 Rat IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1413R Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh  
  Goat PE   1 ml / US$ 455
   
R1428R Rat IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   
R1415R Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SM2017P CCT Theta / TCP1 Theta '.