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SM2019P CCT Eta antibody
see related secondary antibodiessee all 5 CCT Eta products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rat anti CCT Eta PK/16/81a
Synonyms
CCT7 TCP-1-eta MGC110985
Product review
Please read our product review about CCT Eta: Antibodies to CCT.
Product Description
Rat anti CCT Eta PK/16/81a, Presentation: Aff - Purified. Product is tested for Westernblot / Immunoblot ( WB )
Properties
| Applications | Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Bovine ( Bov ), Rabbit ( Rb ) |
| Presentation | Aff - Purified |
| Host | Rat |
| Isotype | IgG2a |
| Clone | PK/16/81a |
| Catalog Number | SM2019P |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM2019P.pdf |
Datasheet Extract
| Monoclonal Rat Antibody to CCT Eta | |
| Concentration | 1.0 mg/ml |
| Background | The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein. |
| Format | State: Liquid purified IgG Purification: Affinity chromatography on Protein G BufferSystem: PBS containing 0.09% Sodium Azide |
| Applications | Western blot: 1/500; detects a band of approximately 60kD in Hela cell lysates.
Immunohistochemistry on frozen sections: 1/100; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Human prostate. |
| Specificity | This antibody recognises the eta polypeptide of the CCT chaperonin molecule complex.
Species: Bovine, Rabbit, Human, Mouse. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Hynes, G. et al. (1996) Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte twodimensional protein database: further characterisation of antibodies to individual subunits. Electrophoresis. 17: 1720-1727.
2. Llorca, O. et al. (2000) Eukaryotic chaperonin CCT stabilizes actin and tubulin folding intermediates in open quasi-native conformations. EMBO J. 19: 5971-5979. 3. Hynes, G.M. Willison, K.R. (2000) Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin. J. Biol. Chem. 275: 18985-18994. |
| Pictures | Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with RAT ANTI CCT ETA (SM2019P) followed by F(ab')2 RABBIT ANTI RAT IgG:HRP (SM2002A). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1371B | Rat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170,00 € | |||||
| R1371F | Rat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1371T | Rat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1371TR | Rat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1371HRP | Rat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1371AP | Rat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 210,00 € | |||||
| R1439C2 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1439C3 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1439C35 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1439C5 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1439C55 | Rat IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1413R | Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1428R | Rat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
| R1415R | Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'SM2019P CCT Eta'.
