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Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD312 (SM2212P).

 

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SM2212P  CD312 / EMR2 antibody

see related secondary antibodies
see all 9 CD312 / EMR2 products
0.2 mg / US$ 555
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Mouse anti CD312 / EMR2 2A1

Synonyms

EGF-Like Module-Containing Mucin-Like Receptor 2, EMR-2, , EGF-Like Module-Containing Mucin-Like Receptor 2, EGF-like Module EMR2

Product review
Please read our product review about CD312 / EMR2: Antibody Panel to proteins of the EGF-Like Module Containing Mucin-Like Hormone Receptor-Like Family.

Product Description

Mouse anti CD312 / EMR2 2A1, Presentation: Purified. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )

Properties

ApplicationsFrozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu )
PresentationPurified
HostMouse
IsotypeIgG1
Clone2A1
Catalog NumberSM2212P
Swiss Prot. No.Q9UHX3
Quantity0.2 mg
PriceUS$ 555
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM2212P.pdf

Datasheet Extract

Alternate namesEMR2
Concentration1.0 mg/ml
BackgroundEMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2.
ImmunogenNIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Swiss Num.: Q9UHX3
Format
State: Liquid purified IgG
Purification: Affinity chromatography on Protein G
BufferSystem: PBS, pH7.4 containing 0.09% Sodium Azide
ApplicationsFlow Cytometry: 1/50 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunoprecipitation.
Western Blot.
Immunohistochemistry on frozen sections.
SpecificityThis antibody recognises EMR2, a member of the epidermal growth factor-seven transmembrane (EGFTM7) family of proteins, which is closely related to CD97. Clone 2A1 specifically recognises the stalk region of EMR2.
Species: Human.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Kwakkenbos. M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol.71:854-862
2. Stacey. M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chrondroitin sulphate glycosaminoglycans. Blood.102:2916-2924
3. Kwakkenbos. M.J. et al. (2004) The EGF-TM7 family: a postgenomic view. Immunogenetics. 55:655-666
PicturesStaining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD312 (SM2212P).

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1253B Mouse IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 305
   
R1253F Mouse IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1253T Mouse IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1253TR Mouse IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1253HRP Mouse IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1253AP Mouse IgG (H&L)  
  Rabbit AP   1 mg / US$ 325
   
R1457C2 Mouse IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1457C3 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1457C35 Mouse IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1457C5 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1457C55 Mouse IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1405R Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   
R1403R Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins  
  Rabbit PE   1 ml / US$ 455
   
R1455R Mouse IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SM2212P CD312 / EMR2'.