SM2251P FCER1A antibody
see related secondary antibodiessee all 31 FCER1A products
0.2 mg / US$ 535
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti FCER1A 9E1
Synonyms
FCER1A, FCE1A, IgE Fc receptor subunit alpha, Fc-epsilon RI-alpha, FcERI, High affinity immunoglobulin epsilon receptor subunit alpha
Product Description
Mouse anti FCER1A 9E1, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Flow cytometry ( F ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | 9E1 |
| Catalog Number | SM2251P |
| Swiss Prot. No. | P12319 |
| Quantity | 0.2 mg |
| Price | US$ 535 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM2251P.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases. |
| Immunogen | CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants. Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line. Swiss Num.: P12319 |
| Format | State: Liquid purified IgG Purification: Affinity chromatography on Protein G BufferSystem: PBS, pH7.4 containing 0.09% Sodium Azide |
| Applications | ELISA.
Western Blot. Immunoprecipitation. Immunofluorescence. Flow Cytometry: 1/25 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. |
| Specificity | This antibody recognises the high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Clone 9E1 specifically recognises the extracellular D1 domain of the Fc epsilon R1 alpha chain. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Vangelista, L. et al. (2002). A minimal receptor-Ig chimera of human Fc epsilon R1 alpha-chain efficiently binds secretory and membrane IgE. Protein Engineering. 15: 51-57.
2. Vangelista, L. et al. (2002) Efficient folding of the Fc epsilonR1 alpha-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1. J. Mol. Biol. 322: 815-25 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM2251P FCER1A'.
