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Staining of mouse peripheral blood platelets with RAT ANTI MOUSE CD321: BIOTIN (SM2256B).

 

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SM2256B  CD321 / JAM1 / F11R antibody

see related secondary antibodies
see all 16 CD321 / JAM1 / F11R products
0.1 mg / US$ 335
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rat anti CD321 / JAM1 / F11R H202-106

Synonyms

F11R, JAM1, JCAM, JAM-1, Platelet adhesion molecule 1, PAM-1, Platelet F11 receptor, CD321 antigen, JAM-A, Junctional adhesion molecule 1, Junctional adhesion molecule A

Product Description

Rat anti CD321 / JAM1 / F11R H202-106, Presentation: Biotin. Product is tested for Frozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP )

Properties

ApplicationsFrozen sections ( C ), Flow cytometry ( F ), Immunoprecipitation ( IP )
ReactivityMouse ( Ms )
PresentationBiotin
HostRat
IsotypeIgG1
CloneH202-106
Catalog NumberSM2256B
Swiss Prot. No.Q9Y624
Quantity0.1 mg
PriceUS$ 335
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SM2256B.pdf

Datasheet Extract

Alternate namesJAM-1
Concentration0.1 mg/ml
BackgroundCD321 is a 32-41kD glycoprotein, which shares similarities with related proteins JAM-2 and JAM-3. CD321 is a multifunctional protein that is primarily expressed by platelets, endothelial and epithelial cells. The CD321 protein co-localises with tight junction molecules in both epithelial and endothelial cells and plays an important role in the regulation of junctional integrity and permeability. In addition, CD321 is a ligand for the integrin LFA-1 and is also involved in the transmigration of leucocytes.
ImmunogenMTE1/MTE2 stromal cell lines. Cells from immunised Lou rats were fused with cells of the X63 mouse myeloma cell line.
Swiss Num.: Q9Y624
Format
State: Liquid purified IgG
Purification: Affinity chromatography on Protein G
BufferSystem: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Label: Biotin
ApplicationsFlow Cytometry: neat - 1/5; use 10 µl of the suggested working dilution to label 106 cells in 100 µl.
SpecificityThis antibody recognises CD321, which is also known as junctional adhesion molecule 1 (JAM-1).
Species: Mouse.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
References1. Malergue, F.et al. (1998) A novel immunoglobulin superfamily junctional molecule expressed by antigen presenting cells, endothelial cells and platelets. Mol. Immunol.35:1111-1119
2. Aurrand-Lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood.98:3699-3707
3. Ebnett, K.et al. (2000) Junctional Adhesion Molecule Interacts with the PDZ Domain-containing Proteins AF-6 and ZO-1. J. Biol. Chem.275:27979-27988
PicturesStaining of mouse peripheral blood platelets with RAT ANTI MOUSE CD321: BIOTIN (SM2256B).

14 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1371B Rat IgG (H&L)  
  Rabbit Biotin   2 mg / US$ 295
   
R1371F Rat IgG (H&L)  
  Rabbit FITC   2 mg / US$ 285
   
R1371T Rat IgG (H&L)  
  Rabbit TRITC   2 mg / US$ 285
   
R1371TR Rat IgG (H&L)  
  Rabbit Texas Red   2 mg / US$ 295
   
R1371HRP Rat IgG (H&L)  
  Rabbit HRP   2 mg / US$ 315
   
R1371AP Rat IgG (H&L)  
  Rabbit AP   1 mg / US$ 335
   
R1439C2 Rat IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1439C3 Rat IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1439C35 Rat IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1439C5 Rat IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1439C55 Rat IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1413R Rat IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Ms, Rb, Sh  
  Goat PE   1 ml / US$ 455
   
R1428R Rat IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   
R1415R Rat IgG F(ab')2 F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh  
  Goat PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SM2256B CD321 / JAM1 / F11R'.