SM297 OX-47 Antigen antibody
see related secondary antibodies
2 ml / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti OX-47 Antigen OX-47
Synonyms
OX44,
Product Description
Mouse anti OX-47 Antigen OX-47, Presentation: Supernatant. Product is tested for Flow cytometry ( F )
Properties
| Applications | Flow cytometry ( F ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Supernatant |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | OX-47 |
| Catalog Number | SM297 |
| Quantity | 2 ml |
| Price | US$ 385 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM297.pdf |
Datasheet Extract
| Alternate names | CD147; Neurothelin |
| Concentration | 12.5 mg/ml |
| Background | OX-47 is expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen is also present on some endothelia. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. It is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.
|
| Immunogen | T blasts from a mixed lymphocyte reaction. Spleen cells from immunised BALB/c mice were fused with cells from the NS0/1 mouse myeloma cell line. |
| Format | State: Liquid Tissue Culture Supernatant BufferSystem: Containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum and 0.09% Sodium Azide |
| Applications | Flow Cytometry: Neat - 1/20; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen sections: Neat - 1/20. |
| Specificity | This antibody recognises a membrane glycoprotein of MW 42-48 kD. Clone OX-47 is reported to be suitable for use in Western blotting. Species: Rat. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Paterson, D.J., Jefferies W.A., Green, J.R., Brandon, M.R., Corthesy, P., Puklavec, M ., Williams, A.F. (1987) Antigens of Activated rat T Lymphocytes including a molecule of 50,000 Mr detected only by CD4 positive T blasts. Mol. Immunol. 24: 1281.
2. Fossum S., Mallett S. & Barclay A.N. (1991) The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence". Eur. J. Immunol. 21: 671. 3. Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels. J. Anat. 189: 407-15. 4. Foda, H. et al. (2001) Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN. Am. J. Respir. Cell. Mol. Biol. 25: 717 - 724. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM297 OX-47 Antigen'.
