SM298B OX-52 Antigen antibody
see related secondary antibodiessee all 5 OX-52 Antigen products
0.1 mg / US$ 275
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti OX-52 Antigen OX-52
Synonyms
OX52, CD6,
Product Description
Mouse anti OX-52 Antigen OX-52, Presentation: Biotin. Product is tested for Frozen sections ( C ), Flow cytometry ( F )
Properties
| Applications | Frozen sections ( C ), Flow cytometry ( F ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Biotin |
| Host | Mouse |
| Isotype | IgG2a |
| Clone | OX-52 |
| Catalog Number | SM298B |
| Quantity | 0.1 mg |
| Price | US$ 275 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM298B.pdf |
Datasheet Extract
| Concentration | 0.1 mg/ml |
| Immunogen | PVG-RT1c thoracic duct lymphocytes and spleen cells.
Donor: BALB/c spleen. Fusion Partner: NSO/U. |
| Format | State: Liquid purified IgG Purification: Protein G Chromatography BufferSystem: PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: Biotin |
| Applications | Flow Cytometry. |
| Specificity | This monoclonal antibody immunoprecipitates a two chain structure (95, 120 kDa) largely restricted to T lymphocytes and thymocytes. Applications therefore include the identification of T lymphocyte lineage cells in suspension and tissue. This antibody has previously been used to stain T cell areas of the spleen, lymph nodes and Peyerâ??s patch (1). In the thymus, this antibody labels all thymocytes, however medullary cells are more strongly positive than cortical cells (1). This Antibody does not label such non-lymphoid tissues such as brain, kidney, liver and skin (1).
This antibody stains approximately 1.0% of bone marrow cells, and 56% thoracic duct lymphocytes. Weak staining occurs with 50% dendritic cells from thoracic duct of mesenteric lymphadenectomized rats. The antigen recognized by this antibody is not expressed on granulocytes or macrophages. The function of the antigen recognized by this antibody has not, as yet, been associated with any particular function of T cells. CL052B does not inhibit the allogeneic mixed leukocyte response, nor does it inhibit T cytotoxic effector cell function. Species: Rat. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Robinson, A.P., Puklavec, M., and D.W. Mason. Immunology. 1986. 57, 527-531. MRC OX-52: A Rat T Cell Antigen. |
| Protocols | FLOW CYTOMETRY ANALYSIS:
Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0.2-0.1 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1:500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: Wistar Cell Concentration: 1x10e6 cells per tests Antibody Concentration Used: 0.1 µg/10e6 cells Isotypic Control: Biotin Mouse IgG2a Cell Source Percentage of cells stained above control: Thymus: 97.2% Spleen: 34.4% Lymph Node: 77.7% |
| Pictures | Cell Source: Thymus - Percentage of cells stained above control: 97.2% |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM298B OX-52 Antigen'.
