SM5004P CAMK2A / CaMK II alpha (pThr286) antibody
see related secondary antibodiessee all 39 CAMK2A / CaMK II alpha products
0.1 ml / US$ 485
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti CAMK2A / CaMK II alpha (pThr286) 22B1
Synonyms
CAMKA, KIAA0968, kinase II subunit alpha, CaMK-II subunit alpha, , Calcium/calmodulin-dependent protein kinase type II alpha chain
Product review
Please read our product review about CAMK2A / CaMK II alpha (pThr286): Antibodies to Calmodulin and CaM-kinases.
Product Description
Mouse anti CAMK2A / CaMK II alpha (pThr286) 22B1, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 22B1 |
| Catalog Number | SM5004P |
| Swiss Prot. No. | P11275 |
| Quantity | 0.1 ml |
| Price | US$ 485 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5004P.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region.
Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation. |
| Immunogen | Synthetic peptide corresponding to residues M(281) H R Q E T(p) V D C L K K F N(294) of rat CaM Kinase II protein. Swiss Num.: P11275 |
| Format | State: Liquid purified IgG fraction. BufferSystem: PBS buffer with 0.05% sodium azide as preservative. |
| Applications | Immunoprecipitation.
ELISA. Immunofluorescence: 1/100, staining of CaM kinase II in rat hippocampal cells with SM5004P densely labels cell somas and neuropil areas, while nuclei are only lightly stained. Western Blot: 1/2000, detects an ~50 kDa band representing the alpha subunit of CaM kinase II only after phosphorylation. |
| Specificity | This antibody detects phosphorylated CaM kinase II from rat tissues.
This antibody has been shown to detect the phosphorylated alpha subunit CaM kinase II only. SM5004P does not detect the non-phosphorylated alpha subunit CaM kinase II nor with the 60 kDa beta subunit in either phosphorylation state. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. J Neuro. 22(10):4196-4204, 2002.
2. Mol. Cell Biol., 21:1444-1452, 2001. 3. J. Neuroscience 24(22):5193-5201, 2004. 4. J. Leukocute Biology Vol. 78, Dec 2005, 5. J. Neuro. Sci. Methods, 68: 61-70, 1996. 6. J. Neurosci., 17(14): 5416-5427, 1997. 7. Science, 280: 1940-1943, 1998. |
| Pictures | Figure 1. Western blot of unphosphorylated and phosphorylated rat brain homogenate, left and right lanes respectively, using SM5004P and SM5005P antibodies. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5004P CAMK2A / CaMK II alpha (pThr286)'.
