SM5038P Rhodopsin Kinase 1a/b antibody
see related secondary antibodiessee all 3 Rhodopsin Kinase 1a/b products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Rhodopsin Kinase 1a/b D11
Synonyms
GRK1a/b, RHOK, , G protein-coupled receptor kinase 1
Product review
Please read our product review about Rhodopsin Kinase 1a/b: Antibodies to Rhodopsin and Rhodopsin Kinases.
Product Description
Mouse anti Rhodopsin Kinase 1a/b D11, Presentation: Purified. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | D11 |
| Catalog Number | SM5038P |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5038P.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events. |
| Immunogen | Full length human GRK1. |
| Format | State: Liquid purified IgG fraction. BufferSystem: PBS with 0.05% sodium azide as preservative. |
| Applications | Immunofluorescence: 1/1000, staining of GRK1a/b in bovine retina with this antibody results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.
Immunohistochemistry on frozen sections: 1/1000. Immunoprecipitation. Western Blot: 1/1000, detects an ~60 kDa protein representing GRK 1a/b. SM5038P detects both in vitro expressed splice variants but was not able to detect significant amounts of GRK1b from human retina. |
| Specificity | This antibody detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues.
This antibody does not cross-react with other G protein-coupled kinases. This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. J. Neurosci., Vol 23: 6152-6160, Jul 2003
2. IOVS, 44 (9): 4076-4085, 2003. 3. FEBS Lett., 454(1-2):115-21, 1999. 4. Ophthalmology, 107(9):1746-53, 2000. 5. IOVS, 44(3):1268-1273, March 2003 6. Invest. Ophthalmol. Vis. Sci., Vol 44: 4076-4085, Sept 2003. |
| Pictures | Figure 1. Immunolocalization of GRK1a/b in bovine retina using SM5038P. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5038P Rhodopsin Kinase 1a/b'.
