SM5039P Rhodopsin antibody
see related secondary antibodiessee all 25 Rhodopsin products
0.1 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Rhodopsin 1D4
Synonyms
RHO, OPN2, Opsin-2, ,
Product review
Please read our product review about Rhodopsin: Antibodies to Rhodopsin and Rhodopsin Kinases.
Product Description
Mouse anti Rhodopsin 1D4, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Human ( Hu ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 1D4 |
| Catalog Number | SM5039P |
| Swiss Prot. No. | P08100 |
| Quantity | 0.1 ml |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5039P.pdf |
Datasheet Extract
| Background | Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain.
The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. |
| Immunogen | Bovine rhodopsin. Swiss Num.: P08100 Remarks: The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope. |
| Format | State: Liquid purified Ig fraction. Purification: Protein G affinity chromatography BufferSystem: PBS containing 1 mg/ml BSA and 0.05% Sodium Azide as preservative. |
| Applications | Immunocytochemistry (1/1,000): Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
Immunoprecipitation. Western blot (1/1,000): This antibody detects an approx. 40 kDa protein representing rhodopsin from Sf9 cells expressing the bovine gene. |
| Specificity | SM5039P detects rhodopsin from bovine, human and rat retinal samples. Species: Human, bovine and rat. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. IOVS 44(11): 4968-4975, 2003.
2. IOVS 44(12):5067- 5074, 2003 3. PNAS, 95:7103-8, 1998. 4. Biochem. J., 320:807-15, 1996. 5. Proc. Natl. Acad. Sci. 95: 7103-7108, 1998 6. Biochem. J. 320: 807-815, 1996 7. JBC, 263(24): 11768-11775, 1988 8. J. Biol. Chem., Mar 2007; 282: 7137 - 7144. 9. Invest. Ophthalmol. Vis. Sci., Apr 2007; 48: 1864 - 1872 10. Hum. Mol. Genet., Vol 12: 1847 - 1863, Aug 2003 11. Invest Ophthalmol Vis Sci. 2007 Apr;48(4):1864-72. 12. JBC, 276(30): 28242-28251, 2001 13. Nature Genetics 20: 344-351, 1998 14. BioChem. 22: 653-660, 1983 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5039P Rhodopsin'.
