SM5055P N-CoR 2 / SMRT antibody
see related secondary antibodiessee all 13 N-CoR 2 / SMRT products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti N-CoR 2 / SMRT 1212
Product Description
Mouse anti N-CoR 2 / SMRT 1212, Presentation: Purified. Product is tested for Frozen sections ( C ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 1212 |
| Catalog Number | SM5055P |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5055P.pdf |
Datasheet Extract
| Monoclonal Antibody to N-CoR 2 (SMRT) | |
| Concentration | 1.0 mg/ml |
| Background | Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity. |
| Immunogen | Synthetic peptide corresponding to residues C G G A(994) S A S D P H R E K T Q(1005) of human SMRT. Remarks: This immunizing peptide (SM5055CP) is available for use in Neutralization and Control experiments. |
| Format | State: Liquid purified IgG fraction. BufferSystem: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative. |
| Applications | Immunohistochemistry: 1/50, staining of SMRT in HeLa cells with SM5055P results in nuclear staining.
Western Blot: 1/250, detects ~250 kDa and ~220 kDa proteins representing endogenous levels of both known isoforms of human SMRT in HeLa cell extracts. |
| Specificity | This antibody detects silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) from human cells. This antibody fails to detect rat SMRT. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Mol. Endocrin., 11: 2025-2037, 1997.
2. Proc. Nat. Acad. Sci. USA, 96(6): 2629-2634, 1999. |
| Pictures | Figure 1. Immunolocalization of SMRT in HeLa cells using SM5055P. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5055P N-CoR 2 / SMRT'.