SM5063 Endothelin 1 / ET-1 antibody
see related secondary antibodiessee all 14 Endothelin 1 / ET-1 products
0.1 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Endothelin 1 / ET-1 TR.ET.48.5
Synonyms
Preproendothelin-1, Big endothelin-1, Endothelin1, ET-1, PPET1, EDN1, ,
Product review
Please read our product review about Endothelin 1 / ET-1 : Antibodies to Endothelin & Endothelin receptors.
Product Description
Mouse anti Endothelin 1 / ET-1 TR.ET.48.5, Presentation: Ascites. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Radioimmunoassay ( R ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Radioimmunoassay ( R ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Rat ( Rt ), Sheep ( Sh ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | TR.ET.48.5 |
| Catalog Number | SM5063 |
| Swiss Prot. No. | P05305 |
| Quantity | 0.1 ml |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5063.pdf |
Datasheet Extract
| Concentration | 2.5 mg/ml |
| Background | Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. |
| Immunogen | Purified human endothelin-1 conjugated to KLH Swiss Num.: P05305 |
| Format | State: Liquid IgG fraction Purification: Affinity chromatography BufferSystem: 1 x PBS containing 0.05% sodium azide |
| Applications | Immunocytochemistry.
Immunohistochemistry on frozen and formalin-fixed, paraffin-embedded tissue sections (1:250): Immunohistochemical staining of ET-1 in human corpus cavernosum tissue results in staining of endothelial cells. Radioimmune assays (1:25,000): can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine). |
| Specificity | SM5063 detects ET-1.
Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16. The antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides. Species: Human, rat, sheep. |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | Mol Human Reproduction Vol 9 No 11, 719-724, Nov 2003.
Circulation 108:1646-1654, 2003. Mol. Hum. Reprod., 8(12): 1053-1064, 2002 Amer. J. Path., 158 (3);841-847, 2001. Amer. J. Path., 157 (2): 1537- 1547, 2000. Am. J. Pathol., Nov 2000; 157: 1537. Am J Physiol Lung Cell Mol Physiol, 278: L981-L987, 2000. Cancer Research, 59: 720-727, 1999. JCEM, 82 (11): 3797-3806, 1997. |
| Pictures | Figure 1 and Figure 2 show immunolocalization of ET-1 in human bowel using antibody SM5063 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5063 Endothelin 1 / ET-1 '.
