SM5094 TGN38 / TGOLN2 antibody
see related secondary antibodiessee all 12 TGN38 / TGOLN2 products
0.1 ml / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti TGN38 / TGOLN2 2F7.1
Synonyms
Golgi Marker, TGN46, TGN48, TGN51, , TGN Marker, Trans-Golgi Network Integral Membrane Protein, Trans-Golgi Network Protein TGN51
Product review
Please read our product review about TGN38 / TGOLN2: Antibody Panel useful as specific Marker for the Golgi Apparatus.
Product Description
Mouse anti TGN38 / TGOLN2 2F7.1, Presentation: Ascites. Product is tested for Flow cytometry ( F ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB )
Properties
| Applications | Flow cytometry ( F ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 2F7.1 |
| Catalog Number | SM5094 |
| Swiss Prot. No. | P19814 |
| Quantity | 0.1 ml |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5094.pdf |
Datasheet Extract
| Background | The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack. The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Some surface receptors recycle between the cell surface and the TGN, suggesting that the TGN is also important in endocytic pathways.
TGN38 and TGN41, an isoform of the former, are integral membrane proteins which are predominantly localized to the TGN in NRK cells but which can also be observed at the cell surface. Complexes which include TGN38, low molecular weight G proteins and p62 are required for the formation of TGN-derived vesicles. This requirement has lead people to believe that TGN38 is important in the trafficking of proteins from the TGN to the cell surface. Brefeldin A causes the contents of the Golgi stack to be redistributed into the endoplasmic reticulum while the TGN collapse upon the microtubule organizing center. This makes immunofluorescence detection of TGN38 following brefeldin A treatment a useful tool for the discrimination of the TGN from the Golgi stack. |
| Immunogen | Synthetic peptide corresponding to amino acid residues 1-17 from rat TGN38 Swiss Num.: P19814 AA Sequence: L(1) P S A S K P N N T S S E N N P P(17)C |
| Format | State: Liquid PBS-diluted ascites containing 0.05% sodium azide |
| Applications | Immunocytochemistry (1/1000).
Immunofluorescence: staining of TGN38 in NRK cells with SM5094 results in staining predominantly in the TGN. In immunofluorescence, it has been shown that methanol works best as a fixative. Flow cytometry. Western Blot (1/500): detects an ~38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal Western blot conditions, this antibody detects an 85-95 kDa band depending on cell type. |
| Specificity | This antibody detects trans-Golgi network (TGN) 38 from both recombinant and endogenous samples. Species: Rat. |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | Mol. Biol. Cell, Sep 2006; 17: 3781-3792.
J. Biol. Chem., Jul 2006; 281: 18888-18900. |
| Pictures | Immunofluorescence staining of TGN-38 (red, Cat. # SM5094) and beta-COP (green, Cat. # SP5139P) in NRK cells. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM5094 TGN38 / TGOLN2'.
