SM5136P O-Linked N-Acetylglucosamine antibody
see related secondary antibodiessee all 7 O-Linked N-Acetylglucosamine products
0.1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti O-Linked N-Acetylglucosamine HGAC85
Synonyms
O-GlcNAc
Product Description
Mouse anti O-Linked N-Acetylglucosamine HGAC85, Presentation: Purified. Product is tested for Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG3 |
| Clone | HGAC85 |
| Catalog Number | SM5136P |
| Quantity | 0.1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM5136P.pdf |
Datasheet Extract
| Monoclonal Antibody to //O-Linked N- Acetylglucosamine (O-GlcNAc) | |
| Concentration | 2.0 mg/ml |
| Background | Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling. |
| Immunogen | Heat killed, pepsin treated group A streptococci. |
| Format | State: Liquid purified Ig fraction. BufferSystem: PBS containing 0.05% sodium azide as preservative. DO NOT USE WITH DILUENTS CONTAINING GLYCOSYLATED PROTEINS. |
| Applications | Western Blot: 1 µg/ml, this antibody detects several proteins representing O-GlcNAc glycoproteins.
Immunoprecipitation. ELISA. Immunofluorescence: 10 µg/ml, staining of O-GlcNAc in cells results in labeling of the nuclear envelope and pores, nucleolus, and cytoplasm. This staining pattern is consistent with other methods of detecting O-GlcNAc moieties. |
| Specificity | SM5136P recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Diabetes Vol. 53, June 2004
2. J. Cell Biol. 104: 1157-1164, 1987. 3. Proc. Natl. Acad. Sci. 57: 5608-5612, 1990. 4. J. Immun., 129(4): 1513-1518, 1982. 5. J. Biol. Chem., 271(49): 31391-31398, 1996. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'SM5136P O-Linked N-Acetylglucosamine'.
