SM7020P Rho GDP-dissociation inhibitor 2 (cleaved) antibody
see related secondary antibodiessee all 35 Rho GDP-dissociation inhibitor 2 products
0.1 mg / US$ 435
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Rho GDP-dissociation inhibitor 2 97A1015
Synonyms
D4-GDI, D4GDI, ARHGDIB, GDIA2, GDID4, RAP1GN1, Ly-GDI, LyGDI, Rho-GDI beta, RhoGDI beta, Rho GDI 2, RhoGDI 2, RhoGDI-2, GDIA-2, GDID-4,
Product Description
Mouse anti Rho GDP-dissociation inhibitor 2 97A1015, Presentation: Purified. Product is tested for Flow cytometry ( F ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Flow cytometry ( F ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 97A1015 |
| Catalog Number | SM7020P |
| Swiss Prot. No. | P52566 |
| Quantity | 0.1 mg |
| Price | US$ 435 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SM7020P.pdf |
Datasheet Extract
| Monoclonal Antibody to D4-GDI | |
| Concentration | 0.5 mg/ml |
| Background | D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms. |
| Immunogen | Synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI Swiss Num.: P52566 |
| Format | State: Liquid purified Ig fraction Purification: Protein G chromatography BufferSystem: PBS containing 0.05% BSA and 0.05% sodium azide |
| Applications | Western blot (2 µg/ml). Recommended positive control: anti-Fas treated Jurkat.
A ~23 kDa band should be observed. Flow cytometry (intracellular, 0.5-1 µg/10e6 cells). Immunohistochemistry (2 µg/ml). Immunoprecipitation (1-2 µg/ml). |
| Specificity | This antibody recognizes D4-GDI. Species: Human, mouse. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Hirohito Yamaguchi, Hong-Gang Wang. Oncogene 20, 7779-7786 (2001).
2. Saori Sato et al., Oncogene 21, 1727-1738 (2002). 3. John J. Gildea et al., Cancer Res. 62: 6418-6423 (2002). 4. Hirohito Yamaguchi et al., Cancer Res., 62: 466-471 (2002). 5. Sandy M. Cuddeback et al., J. Biol. Chem, 276: 20559-20565 (2001). 6. Yen-Chou Chen et al., Biochemical Pharmacology 64: 1713-1724 (2002). 7. Yen-Chou Chen et al., Biochem Pharmacol, 66(7): 1139-1150 (2003). 8. Shing-Chuan Shen et al., J Cell Biochem, 89: 1044-1055 (2003). 9. Chun-Yi Liu et al., Ann. N.Y. Acad. Sci 1011: 133 - 145 (2004). 10. Christopher E. Jenkins et al., J. Biol. Chem., 279: 37201-37207 (2004). Ching Huai Ko et al., Free Radical Biology & Medicine, 36 (7): 897-910 (2004). |
| Pictures | Western blot analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb. Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI cleavage was detected by SM7020P.
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SM7020P Rho GDP-dissociation inhibitor 2 '.