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Immunohistochemical staining of Brain (Pituitary) using anti- GPR12 antibody SP4187P

 

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SP4187P  G-protein coupled receptor 12 antibody

see related secondary antibodies
see all 3 G-protein coupled receptor 12 products
50 µg / no delivery possible
please contact the manufacturer

Quick Overview

Rabbit anti G-protein coupled receptor 12

Synonyms

GPR12,

Product Description

Rabbit anti G-protein coupled receptor 12 , Presentation: Aff - Purified. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )

Properties

ApplicationsParaffin Sections ( P ), Western blot / Immunoblot ( WB )
ReactivityHuman ( Hu ), Mouse ( Ms )
PresentationAff - Purified
HostRabbit
Catalog NumberSP4187P
Swiss Prot. No.P47775
Quantity50 µg
Priceno delivery possible
DeliveryNot USA/Canada
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SP4187P.pdf

Datasheet Extract

Alternate namesMGC138349, MGC138351, GPCR12, GPCR21, g protein-coupled receptor 12, gpcr21, gpr12
Concentration1 mg/ml
BackgroundGPR12 has been identified as a constitutively active Lysophospholipid/Lysosphingolipid receptor.
GPR12 expression has been reported primarily in the central nervous system. Rat transcripts have been identified at low levels in testis and pituitary gland. ESTs have been isolated from human brain and eye libraries, as well as mouse brain, genitourinary tract, and liver libraries.
Ligand is Sphingosine-1-phosphate (SPP)
Caution: GPR12 is distinct from GPR12A, also known as OGR1, in cluster 3870.
ImmunogenSynthetic peptide - KLH conjugated
Epitope: C-terminal domain of human
Genename: GPR12
Swiss Num.: P47775
Remarks: The immunizing peptide shows 93 % identity (100 % similarity) with mouse.
Format
State: Liquid Ig fraction
Purification: Peptide immunogen affinity column
BufferSystem: PBS, pH 7.7, containing 0.01% sodium azide
ApplicationsWestern Blot.
Immunohistochemistry with formalin fixed paraffin embedded tissues: 2-4 µg/ml
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
SpecificityThis antibody reacts with G Protein-Coupled Receptor GPR12
(family: GPCR; subfamily: Lysophospholipid/Lysosphingolipid)


Species: Human, Mouse.
Add. InformationThis product was originally produced by MBL International.
StorageStore the antibody undiluted at 2-8°C for one month or (in aliquots) at -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
PicturesImmunohistochemical staining of Brain (Pituitary) using anti- GPR12 antibody SP4187P

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SP4187P G-protein coupled receptor 12'.