SP5141P Early endosome antigen 1 antibody
see related secondary antibodiessee all 15 Early endosome antigen 1 products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Early endosome antigen 1
Synonyms
EEA1, ZFYVE2, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
Product Description
Rabbit anti Early endosome antigen 1 , Presentation: Aff - Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Canine ( Can ), Hamster ( Hst ), Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP5141P |
| Swiss Prot. No. | Q15075 |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5141P.pdf |
Datasheet Extract
| Polyclonal Antibody to Early Endosome Antigen 1 | |
| Concentration | 1.0 mg/ml |
| Background | Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes |
| Immunogen | Synthetic Peptide corresponding aa 1391 - 1410 of human EEA1 Swiss Num.: Q15075 AA Sequence: T(1391) P S S K K P V R V C D A C F N D L Q G(1410) Remarks: SP5141P immunizing peptide (SP5141CP) is available for use in neutralization and control experiments. |
| Format | State: Liquid purified IgG fraction. Purification: Immunoaffinity chromatography. BufferSystem: PBS containing 1 mg/ml BSA as stabilizer and 0.05% Sodium Azide as preservative. |
| Applications | Immunofluorescence: 4 µg/ml, yields a punctate staining pattern in HeLa cells consistent with the cytoplasmic distribution of endosomes.
Western Blot: 1 µg/ml, detects an ~160 kDa protein representing EEA1 from mouse brain extract. Immunoprecipitation (human and mouse). |
| Specificity | Highly specific for EAA1. Species: Human, mouse, hamster, canine and rat. |
| Storage | Aliquot and store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | Invest. Ophthalmol. Vis. Sci. 2005 46: 1882-1890.
|
| Pictures | Figure 1 Immunolocalization of EEA1 in HeLa cells using SP5141P. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5141P Early endosome antigen 1 '.
